Difference between revisions of "Part:BBa K143073"

 
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<partinfo>BBa_K143073 short</partinfo>
 
<partinfo>BBa_K143073 short</partinfo>
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AmyE 5' Integration sequence(<bbpart>BBa_K143001</bbpart>) coupled to a chloramphenicol resistance generator in closed transcriptional unit (Parts <bbpart>BBa_K143052</bbpart> and <bbpart>BBa_K143064</bbpart>) and the PoPS generator Pveg-gsiB (<bbpart>BBa_K143052</bbpart>).
 
AmyE 5' Integration sequence(<bbpart>BBa_K143001</bbpart>) coupled to a chloramphenicol resistance generator in closed transcriptional unit (Parts <bbpart>BBa_K143052</bbpart> and <bbpart>BBa_K143064</bbpart>) and the PoPS generator Pveg-gsiB (<bbpart>BBa_K143052</bbpart>).
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The amyE 5' integration sequence allows integration into the ''B. subtilis'' genome at the amyE locus if the 3' amyE integration sequence(<bbpart>BBa_K143002</bbpart>) is cloned onto the 3' end of the construct.
 
The amyE 5' integration sequence allows integration into the ''B. subtilis'' genome at the amyE locus if the 3' amyE integration sequence(<bbpart>BBa_K143002</bbpart>) is cloned onto the 3' end of the construct.
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The chloramphenicol adenyltransferase give resistance to chloramphenicol while the terminator prevents readthrough.
 
The chloramphenicol adenyltransferase give resistance to chloramphenicol while the terminator prevents readthrough.
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The Pveg-gsiB promoter and RBS for ''B. subtilis'' constitutively generate a PoPS output.
 
The Pveg-gsiB promoter and RBS for ''B. subtilis'' constitutively generate a PoPS output.
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><big>'''Sequence and Features'''</big></span>
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<partinfo>BBa_K143073 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143073 SequenceAndFeatures</partinfo>
  

Latest revision as of 16:48, 27 October 2008

AmyE integratable PoPS generator (Pveg-gsiB) (with CmR)


AmyE 5' Integration sequence(BBa_K143001) coupled to a chloramphenicol resistance generator in closed transcriptional unit (Parts BBa_K143052 and BBa_K143064) and the PoPS generator Pveg-gsiB (BBa_K143052).


The amyE 5' integration sequence allows integration into the B. subtilis genome at the amyE locus if the 3' amyE integration sequence(BBa_K143002) is cloned onto the 3' end of the construct.


The chloramphenicol adenyltransferase give resistance to chloramphenicol while the terminator prevents readthrough.


The Pveg-gsiB promoter and RBS for B. subtilis constitutively generate a PoPS output.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]