Difference between revisions of "Part:BBa K3515008:Design"
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===References=== | ===References=== | ||
+ | Shen, Y., Wu, S.Y., Rancic, V., Aggarwal, A., Qian, Y., Miyashita, S.I., Ballanyi, K., Campbell, R.E. and Dong, M., 2019. Genetically encoded fluorescent indicators for imaging intracellular potassium ion concentration. Communications biology, 2(1), pp.1-10. |
Revision as of 13:48, 25 May 2020
Potassium binding protein with cysteine modification(s) to bind to a biosensor and FRET to monitor p
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 766
Illegal AgeI site found at 823
Illegal AgeI site found at 883 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added a cysteine for immobilization (A26C) opposite to the active site region for linker arm immobilization. Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation.
Source
The source of this part is its sequence retrieved from the European Nucleotide Archive (U00096.3) along with our own modifications.
References
Shen, Y., Wu, S.Y., Rancic, V., Aggarwal, A., Qian, Y., Miyashita, S.I., Ballanyi, K., Campbell, R.E. and Dong, M., 2019. Genetically encoded fluorescent indicators for imaging intracellular potassium ion concentration. Communications biology, 2(1), pp.1-10.