Difference between revisions of "Part:BBa K3122006"
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Ptac, trp and lac regulated promoter (Part:BBa_K864400), adapted to MoClo standard by our team. It was designed to control Streptavidin-CBDs expression via IPTG induction.  
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Ptac, trp and lac regulated promoter (Part: <partinfo>BBa_K864400</partinfo>), adapted to MoClo standard by our team. It was designed to control Streptavidin-CBDs expression via IPTG induction.  
  
 
<h2> Description </h2>
 
<h2> Description </h2>
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<h2> Characterization </h2>
 
<h2> Characterization </h2>
 
   
 
   
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Characterization of this part was planned to be conducted by building three transcriptional units (<partinfo>BBa_K3122017</partinfo>, <partinfo>BBa_K3122018</partinfo>, <partinfo>BBa_K31220179</partinfo>) expressing different recombinant proteins. Unfortunately, no results were obtained.
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Characterization of this part was planned to be conducted by building three transcriptional units (<partinfo>BBa_K3122017</partinfo>, <partinfo>BBa_K3122018</partinfo>, <partinfo>BBa_K3122019</partinfo>) expressing different recombinant proteins. Unfortunately, no results were obtained.
 
   
 
   
  

Revision as of 03:18, 22 October 2019


Inducible Ptac promoter (TypeIIS adapted)


Ptac, trp and lac regulated promoter (Part: BBa_K864400), adapted to MoClo standard by our team. It was designed to control Streptavidin-CBDs expression via IPTG induction.

Description

Promotor Ptac is a combination of the different promoters from the lac and tpr operons. The advantages of this promoter include its high efficiency (11 times more than the lac one and 3 times more than the tpr one) and the fine control of the expression it allows (it can be repressed by lac repressor and derepressed with isopropyl-beta-D-thiogalactoside).

Characterization

Characterization of this part was planned to be conducted by building three transcriptional units (BBa_K3122017, BBa_K3122018, No part name specified with partinfo tag.) expressing different recombinant proteins. Unfortunately, no results were obtained.


References

de Boer, H., Comstock, L. and Vasser, M. (1983). The tac promoter: a functional hybrid derived from the trp and lac promoters. Proceedings of the National Academy of Sciences, 80(1), pp.21-25.