Difference between revisions of "Part:BBa K2910002"
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Revision as of 03:12, 22 October 2019
IsPETase-ML Mut 1 with C-terminal Hexahistidine Tag
BBa_K2910002 encodes a variant of the Ideonella sakaiensis PETase, optimized for E. coli, which degrades poly(ethylene terephthalate. Using a Machine Learning Algorithm to optimize hydrophobicity content, the thermostability of the protein is predicted to be improved.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
<img src="https://parts.igem.org/File:T--Toronto--pNPB-Hydrolysis-pH-8_Tianyu-All-Conc.png">
<img src="https://parts.igem.org/File:T--Toronto--pNPB-Hydrolysis-pH-9_Tianyu-All-Conc.png">