Difference between revisions of "Part:BBa K2966006"
| Line 4: | Line 4: | ||
This part is the constitutive promoter, Pc along with the codon-optimized NodC for P. fluorescens and finally the T4 transcriptional terminator. | This part is the constitutive promoter, Pc along with the codon-optimized NodC for P. fluorescens and finally the T4 transcriptional terminator. | ||
| + | |||
| + | <h2>Characterization</h2> | ||
| + | https://2019.igem.org/wiki/images/b/b8/T--Purdue--S3controlS4.jpg | ||
| + | The figure above shows Pc with NodC_Pf, a negative control, and PpsbA with NodC_Pf. The negative control was used to show that the wildtype E. coli does not normally fluoresce. Pc with NodC_Pf had almost all of its cells fluoresce blue, and PpsbA with NodC_Pf had most of its cells fluoresce as well. | ||
| + | |||
[[File:T--Purdue--s4overlay.jpg|50px]] | [[File:T--Purdue--s4overlay.jpg|50px]] | ||
Revision as of 03:38, 22 October 2019
PpsbA-NodC_Pf-T4 term
This part is the constitutive promoter, Pc along with the codon-optimized NodC for P. fluorescens and finally the T4 transcriptional terminator.
Characterization
The figure above shows Pc with NodC_Pf, a negative control, and PpsbA with NodC_Pf. The negative control was used to show that the wildtype E. coli does not normally fluoresce. Pc with NodC_Pf had almost all of its cells fluoresce blue, and PpsbA with NodC_Pf had most of its cells fluoresce as well.
50px
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]