Difference between revisions of "Part:BBa K2910003"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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https://static.igem.org/mediawiki/parts/0/06/T--Toronto--pNPB-Hydrolysis-pH-8_Dimi-2500uM.png | https://static.igem.org/mediawiki/parts/0/06/T--Toronto--pNPB-Hydrolysis-pH-8_Dimi-2500uM.png | ||
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https://static.igem.org/mediawiki/parts/7/7d/T--Toronto--pNPB-Hydrolysis-pH-8_Dimi-156uM.png | https://static.igem.org/mediawiki/parts/7/7d/T--Toronto--pNPB-Hydrolysis-pH-8_Dimi-156uM.png | ||
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Revision as of 02:53, 22 October 2019
IsPETase-T88F-I208Y-S238F-S245Y-R280A with C-terminal Hexahistidine Tag
This part encodes Ideonella sakaiensis poly(ethylene terephthalate) hydrolase with several mutations predicted to increase its catalytic activity. The bulky arginine residue at 280 has been mutated to an alanine to reduce steric clash with PET. The other mutations are predicted to increase interaction between isPETase and aromatic moieties of PET.
Internal working name: Dimi1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 67
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]