Difference between revisions of "Part:BBa K1223006:Experience"

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<partinfo>BBa_K1223006 AddReview number</partinfo>
<partinfo>BBa_K1223006 review by MADRID_UCM 2019 team</partinfo>
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<h2> Characterization of <partinfo>BBa_K1223006</partinfo> by 2019 MADRID_UCM team</h2>
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Characterization of this part has been conducted by building a transcriptional unit <partinfo>BBa_K3122004</partinfo> and performing assays with nickel beads in order to check the interaction between them and its force. This transcriptional unit was assembled in pARK1 alpha plasmid including the following parts:
 
Characterization of this part has been conducted by building a transcriptional unit <partinfo>BBa_K3122004</partinfo> and performing assays with nickel beads in order to check the interaction between them and its force. This transcriptional unit was assembled in pARK1 alpha plasmid including the following parts:
 
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Revision as of 01:55, 22 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1223006

User Reviews

UNIQ7d43efd62d938fff-partinfo-00000000-QINU

No review score entered. franquero

Characterization of BBa_K1223006 by 2019 MADRID_UCM team

Characterization of this part has been conducted by building a transcriptional unit BBa_K3122004 and performing assays with nickel beads in order to check the interaction between them and its force. This transcriptional unit was assembled in pARK1 alpha plasmid including the following parts:

  • BBa_K3122003: Promoter J23107 adapted to type IIS assembly
  • BBa_K2656009: RBS  B0030 adapted to type IIS assembly
  • BBa_K3122002: coding sequence. This composite part is built with BBa_K3122000 (LamB protein) and BBa_K3122001 (6xHis tag) creating our AEGIS' lamB display system exposing 6xHis Tag.
  • BBa_K2656026: Terminator B0015 adapted to type IIS assembly
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UNIQ7d43efd62d938fff-partinfo-00000008-QINU