Difference between revisions of "Part:BBa K2970002"

Revision as of 01:36, 22 October 2019 (view source) MarieTT (Talk | contribs) ← Older edit		Latest revision as of 01:37, 22 October 2019 (view source) MarieTT (Talk | contribs)
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	===Results===		===Results===
−	For results visit page of <partinfo>BBa_K2980006</partinfo>.	+	For results visit page of <partinfo>BBa_K2980006</partinfo>. <br>
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	<!-- Add more about the biology of this part here		<!-- Add more about the biology of this part here
	===Usage and Biology===		===Usage and Biology===

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Latest revision as of 01:37, 22 October 2019

Gate

This gate is a toehold switch system with which a gene of interest can be locked and regulated on a translational level using mRNA as regulator. After transcription, the mRNA of this gate forms a hairpin structure that hides the ribosome binding site and start codon of the gene of interest, thus translation can not be initiated (Figure 1B). A complementary part to the gate, a so called trigger, is needed to open the hairpin and release the ribosome binding site. In our case two triggers are needed that form a trigger complex to open the gate (BBa_K2970000 and BBa_K2970001). The affinity between the trigger complex and the gate is greater than that of the gate to itself (in the hairpin). A single trigger cannot open the gate because it has only half the required complementary sequence.

To transform this part into bacteria it should be put on a plasmid together with a promoter and a terminator. We used this part to regulate a chloramphenicol resistance gene that we put behind the gate (BBa_K2970006).

Results

For results visit page of BBa_K2980006.

Sequence and Features