Difference between revisions of "Part:BBa K3019009"
 
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Pre-Ost1-pro-alfa-factor signal promotes translocation to the ER during translation. Arthrobacter sp. YCWD3 glcI gene for beta-1,3-glucanase, complete cds. Codon optimised for expression in S. cerevisiae. This glucanase is a part of Zymolyaze.
 
Pre-Ost1-pro-alfa-factor signal promotes translocation to the ER during translation. Arthrobacter sp. YCWD3 glcI gene for beta-1,3-glucanase, complete cds. Codon optimised for expression in S. cerevisiae. This glucanase is a part of Zymolyaze.
  
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[[File:T--Tartu TUIT--Westernblot.png|500px]] <br>
 
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<b>Figure.</b> Western Blot analysis to test the expression and secretion of Glc1. Western blot image showing the expression of α-3HA-Glc1 and Ost1-3HA-Glc1. The image shows the amount of the protein in cell lysate. The lysate was diluted 20 and 40 times to visualize the two fragments and to avoid signal leak to neighbouring lanes.
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3019009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3019009 SequenceAndFeatures</partinfo>
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[[File:T--Tartu TUIT--Westernblot.png|500px]] <br>
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<b>Figure.</b> Western Blot analysis to test the expression and secretion of Glc1. Western blot image showing the expression of α-3HA-Glc1 and Ost1-3HA-Glc1. The image shows the amount of the protein in cell lysate. The lysate was diluted 20 and 40 times to visualize the two fragments and to avoid signal leak to neighbouring lanes.
  
  

Latest revision as of 01:25, 22 October 2019


Pre-Ost1-pro-α-factor signal 3HA GLCI

Pre-Ost1-pro-alfa-factor signal promotes translocation to the ER during translation. Arthrobacter sp. YCWD3 glcI gene for beta-1,3-glucanase, complete cds. Codon optimised for expression in S. cerevisiae. This glucanase is a part of Zymolyaze.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 645
    Illegal AgeI site found at 1726
  • 1000
    COMPATIBLE WITH RFC[1000]

T--Tartu TUIT--Westernblot.png
Figure. Western Blot analysis to test the expression and secretion of Glc1. Western blot image showing the expression of α-3HA-Glc1 and Ost1-3HA-Glc1. The image shows the amount of the protein in cell lysate. The lysate was diluted 20 and 40 times to visualize the two fragments and to avoid signal leak to neighbouring lanes.