Difference between revisions of "Part:BBa K3292001"
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The characterization of this part included a Bradford assay to quantify the amount of protein present in the medium for each of the proposed constructs. | The characterization of this part included a Bradford assay to quantify the amount of protein present in the medium for each of the proposed constructs. | ||
Revision as of 01:16, 22 October 2019
T7-LacO Promoter + strong RBS + sialidase + sfGFP + 6x his-tag + double terminator
This device uses Bba_K2406020, Bba_J34801 and Bba_0010 to regulate the expression of a super folding GFP (Bba_I746916). The main objective of creating this device is to facilitate the secretion and characterization of any protein intracellularly produced after adding its nucleotide sequence next to the sfGFP.
<Usage and Biology The characterization of this part included a Bradford assay to quantify the amount of protein present in the medium for each of the proposed constructs.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 721
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1986
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 646
Illegal BsaI.rc site found at 510
Illegal BsaI.rc site found at 1647
Illegal SapI.rc site found at 2055