Difference between revisions of "Part:BBa K2935073"

Revision as of 01:08, 22 October 2019

Flo1

BBa_K2935073 (677bp) in saccharomyces cerevisiae chromosome S288C I position 205985-206661.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 121
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 121
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 121
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 121
Illegal AgeI site found at 25
Illegal AgeI site found at 67
Illegal AgeI site found at 202
Illegal AgeI site found at 472
1000
COMPATIBLE WITH RFC[1000]

 In the genome of saccharomyces cerevisiae, most of the genes contain repeated sequences. Verstrepen,K.J. et al. [1] compared its effect on eight newly generated FLO1 alleles.It was found that as the Flo1 protein became longer (carrying more repeated sequences), the adhesion increased.Flocculation (i.e., adhesion to other yeast cells) showed a quantitative relationship between the same number of repetitions and the number of repetitions: the more repetitions, the greater the proportion of flocculated cells.The correlation between the number of repetitions observed and functional acquisition Flo1 depends on the specific amino acid encoded by the repeat sequence.
 To look for in-gene duplication, they used EMBOSS ETANDEMsoftware26 to screen all sacs ORFs sequences.Two separate screens screened short (3-39 nt) and long (440 nt) repeats, respectively.

They ended up with BBa_K2935073 (677bp) as the basis and common piece of all FLO1.

REFERENCE:Intragenic tandem repeats generate functional variability.Verstrepen,K.J., Jansen,A., Lewitter,F. and Fink,G.R. Nat. Genet. 37 (9), 986-990 (2005)

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 <partinfo>BBa_K2935073 short</partinfo>
-Flo1
+BBa_K2935073 (677bp) in saccharomyces cerevisiae chromosome S288C I position 205985-206661.
 <!-- Add more about the biology of this part here
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 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2935073 SequenceAndFeatures</partinfo>
-BBa_K2935073 (677bp) in saccharomyces cerevisiae chromosome S288C I position 205985-206661.
-In the genome of saccharomyces cerevisiae, most of the genes contain repeated sequences. Verstrepen,K.J. et al. [1] compared its effect on eight newly generated FLO1 alleles.It was found that as the Flo1 protein became longer (carrying more repeated sequences), the adhesion increased.Flocculation (i.e., adhesion to other yeast cells) showed a quantitative relationship between the same number of repetitions and the number of repetitions: the more repetitions, the greater the proportion of flocculated cells.The correlation between the number of repetitions observed and functional acquisition Flo1 depends on the specific amino acid encoded by the repeat sequence.
+  In the genome of saccharomyces cerevisiae, most of the genes contain repeated sequences. Verstrepen,K.J. et al. [1] compared its effect on eight newly generated FLO1 alleles.It was found that as the Flo1 protein became longer (carrying more repeated sequences), the adhesion increased.Flocculation (i.e., adhesion to other yeast cells) showed a quantitative relationship between the same number of repetitions and the number of repetitions: the more repetitions, the greater the proportion of flocculated cells.The correlation between the number of repetitions observed and functional acquisition Flo1 depends on the specific amino acid encoded by the repeat sequence.
-To look for in-gene duplication, they used EMBOSS ETANDEMsoftware26 to screen all sacs ORFs sequences.Two separate screens screened short (3-39 nt) and long (440 nt) repeats, respectively.
+  To look for in-gene duplication, they used EMBOSS ETANDEMsoftware26 to screen all sacs ORFs sequences.Two separate screens screened short (3-39 nt) and long (440 nt) repeats, respectively.
 They ended up with BBa_K2935073 (677bp) as the basis and common piece of all FLO1.