Difference between revisions of "Part:BBa K3189001"

Revision as of 00:35, 22 October 2019

Phage Lambda-TetO

A modified phage lambda PL promoter with tet operator sites. This part functions as a promoter that allows for tetracycline-based transcriptional activation

The flasks in Figure 1 shows 50 mL E.coli cells transformed with gfp under the control of BBa_K3189001. On the plasmid containing BBa_K3189001 is the repressor, BBa_K3189004, needed for proper function of the promoter. Tetracycline was used to induce the system, resulting in GFP production and fluorescence is seen using a UV wand.


Figure 1: E. coli cells expressing GFP under the control of BBa_K3189001 induced with tetracycline.


An initial expression test of GFP under the control of BBa_K3189001 was preformed using 1 ng/mL and 50 ng/mL tetracycline. It can be seen in Figure 2 that more fluorescence was seen with the higher concentration of tetracycline. Based on these results it seems that BBa_K3189001 responds to tetracycline in a dose dependent manner.


Figure 2: E. coli strains DH5a and BL21(DE3) expressing GFP under the control of BBa_K3189001 using 1 ng/mL and 50 ng/mL tetracycline.

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Sequence and Features