Difference between revisions of "Part:BBa K3189001"
m |
m |
||
| Line 7: | Line 7: | ||
<br> | <br> | ||
The flasks in Figure 1 shows 50 mL <i>E.coli</i> cells transformed with <i>gfp</i> under the control of BBa_K3189001. On the plasmid containing BBa_K3189001 is the repressor, BBa_K3189004, needed for proper function of the promoter. Tetracycline was used to induce the system, resulting in GFP production and fluorescence is seen using a UV wand. | The flasks in Figure 1 shows 50 mL <i>E.coli</i> cells transformed with <i>gfp</i> under the control of BBa_K3189001. On the plasmid containing BBa_K3189001 is the repressor, BBa_K3189004, needed for proper function of the promoter. Tetracycline was used to induce the system, resulting in GFP production and fluorescence is seen using a UV wand. | ||
| + | <br> | ||
<br> | <br> | ||
https://static.igem.org/mediawiki/parts/8/86/T--Guelph--tetO_characterization.JPG | https://static.igem.org/mediawiki/parts/8/86/T--Guelph--tetO_characterization.JPG | ||
<br> | <br> | ||
| − | + | <b>Figure 1: E. coli cells expressing GFP under the control of BBa_K3189001 induced with tetracycline.</b> | |
<br> | <br> | ||
https://static.igem.org/mediawiki/parts/5/50/T--Guelph--GPRexpression.PNG | https://static.igem.org/mediawiki/parts/5/50/T--Guelph--GPRexpression.PNG | ||
Revision as of 00:34, 22 October 2019
Phage Lambda-TetO
A modified phage lambda PL promoter with tet operator sites. This part functions as a promoter that allows for tetracycline-based transcriptional activation
The flasks in Figure 1 shows 50 mL E.coli cells transformed with gfp under the control of BBa_K3189001. On the plasmid containing BBa_K3189001 is the repressor, BBa_K3189004, needed for proper function of the promoter. Tetracycline was used to induce the system, resulting in GFP production and fluorescence is seen using a UV wand.
Figure 1: E. coli cells expressing GFP under the control of BBa_K3189001 induced with tetracycline.
text
text
text
text
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]