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Revision as of 00:31, 22 October 2019


PETase (PET-degrading enzyme, origin I. sakaiensis)

PETase is a poly(ethylene terehphthalate)-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.

T--Toronto--pNPB-Hydrolysis-pH-7_Wildtype-All-Conc.png T--Toronto--pNPB-Hydrolysis-pH-8-Wildtype-All-Conc.png T--Toronto--pNPB-Hydrolysis-pH-9_Wildtype-All-Conc.png T--Toronto--pNPB-Hydrolysis-Wildtype_pH7-8-9.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 67
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]