Difference between revisions of "Part:BBa K3269004"
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<partinfo>BBa_K3269004 short</partinfo> | <partinfo>BBa_K3269004 short</partinfo> | ||
| − | This part includes having a LexA 1 operator upstream of GFP. This part was created with the intention of improving part [[Part:BBa_K079050|BBa_K079050]]. For more information about our experience using this part, please view the experience page and the page for part BBa_K079050. | + | This part includes having a LexA 1 operator upstream of GFP. This part was created with the intention of improving part [[Part:BBa_K079050|BBa_K079050]]. For more information about our experience using this part and how the LexA 1 operator compares to the LexA 2 operator, please view the experience page and the page for part BBa_K079050. |
This part was meant to produce GFP proportionally to the amount of UV exposure. It was supposed to be a UV DNA damage sensor. However, it did not work as we anticipated. As our results in Figure 1 show, there was no significant difference in fluorescence level when the amount of UV changed. | This part was meant to produce GFP proportionally to the amount of UV exposure. It was supposed to be a UV DNA damage sensor. However, it did not work as we anticipated. As our results in Figure 1 show, there was no significant difference in fluorescence level when the amount of UV changed. | ||
https://static.igem.org/mediawiki/parts/b/be/T--Northwestern--lexA1data.png <br> Figure 1. Fluorescence/OD for different levels of UV for part BBa_K3269004 | https://static.igem.org/mediawiki/parts/b/be/T--Northwestern--lexA1data.png <br> Figure 1. Fluorescence/OD for different levels of UV for part BBa_K3269004 | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 23:41, 21 October 2019
LexA1 GFP composite part
This part includes having a LexA 1 operator upstream of GFP. This part was created with the intention of improving part BBa_K079050. For more information about our experience using this part and how the LexA 1 operator compares to the LexA 2 operator, please view the experience page and the page for part BBa_K079050.
This part was meant to produce GFP proportionally to the amount of UV exposure. It was supposed to be a UV DNA damage sensor. However, it did not work as we anticipated. As our results in Figure 1 show, there was no significant difference in fluorescence level when the amount of UV changed.
Figure 1. Fluorescence/OD for different levels of UV for part BBa_K3269004
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 729