Difference between revisions of "Part:BBa K3002101"
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This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>, an HA-tag (<a href="https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>)and the coding sequence of the mutant PETase, Mutate M, of the iGEM team TJUSLS China (<a href="https://parts.igem.org/Part:BBa_K1921003">BBa_K1921003</a>)plus the MoClo connectors for positions B2-B3 ( <a href="https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>), B4-B5 ( <a href="https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>) and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>). | This composite part contains the PAR-promoter (<a href="https://parts.igem.org/Part:BBa_K3002027">BBa_K3002027</a>) in combination with the RPL23-Terminator (<a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>, an HA-tag (<a href="https://parts.igem.org/Part:BBa_K3002017">BBa_K3002017</a>)and the coding sequence of the mutant PETase, Mutate M, of the iGEM team TJUSLS China (<a href="https://parts.igem.org/Part:BBa_K1921003">BBa_K1921003</a>)plus the MoClo connectors for positions B2-B3 ( <a href="https://parts.igem.org/Part:BBa_K3002303">BBa_K3002303</a>), B4-B5 ( <a href="https://parts.igem.org/Part:BBa_K3002304">BBa_K3002304</a>) and B5-B6 (<a href="https://parts.igem.org/Part:BBa_K3002305">BBa_K3002305</a>). | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 23:38, 21 October 2019
Level 1 Mutant PETase + HA (Phytobrick)
This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006, an HA-tag (BBa_K3002017)and the coding sequence of the mutant PETase, Mutate M, of the iGEM team TJUSLS China (BBa_K1921003)plus the MoClo connectors for positions B2-B3 ( BBa_K3002303), B4-B5 ( BBa_K3002304) and B5-B6 (BBa_K3002305).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 249
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1722
- 1000COMPATIBLE WITH RFC[1000]