Difference between revisions of "Part:BBa K1653002"
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https://2019.igem.org/wiki/images/1/1c/T--SEFLS_Shanghai--BBa_K1653002-fig1.jpg | https://2019.igem.org/wiki/images/1/1c/T--SEFLS_Shanghai--BBa_K1653002-fig1.jpg | ||
<p>Genes idi and ispA were added to the plasmid pET-YSS, yielding pET-IAY. Co-transformation of the plasmid pET-IAY with p35151 resulted in squalene production of 69.3 mg/L (strain H2), an approximately 3.7-fold increase compared to BL21(DE3) harboring pET-YSS and p35151(Figure. 2). This result indicated that the overexpression of idi and ispA increased the supply of precursor, and thus increasing the yield of squalene. </p> | <p>Genes idi and ispA were added to the plasmid pET-YSS, yielding pET-IAY. Co-transformation of the plasmid pET-IAY with p35151 resulted in squalene production of 69.3 mg/L (strain H2), an approximately 3.7-fold increase compared to BL21(DE3) harboring pET-YSS and p35151(Figure. 2). This result indicated that the overexpression of idi and ispA increased the supply of precursor, and thus increasing the yield of squalene. </p> | ||
| − | https://2019.igem.org/wiki/images/0/07/T--SEFLS_Shanghai--BBa_K1653002-fig2.jpg | + | <img src="https://2019.igem.org/wiki/images/0/07/T--SEFLS_Shanghai--BBa_K1653002-fig2.jpg"></img> |
<p>Figure.2 construction of strains H1, H2 & H3</p> | <p>Figure.2 construction of strains H1, H2 & H3</p> | ||
<p> Compare plasmids pETDuet-1T7-YSS-2T7-CrtN <strong>BBa_K3166998 </strong>with<strong> </strong>pETDuet-rbs-idi-rbs-ispA-rbs-YSS-2T7-CrtN <strong>BBa_K3166808</strong></p> | <p> Compare plasmids pETDuet-1T7-YSS-2T7-CrtN <strong>BBa_K3166998 </strong>with<strong> </strong>pETDuet-rbs-idi-rbs-ispA-rbs-YSS-2T7-CrtN <strong>BBa_K3166808</strong></p> | ||
Revision as of 22:49, 21 October 2019
idi( isopentenyl-diphosphate delta-isomerase)
Isopentenyl diphosphate isomerase encodes Isopentenyl diphosphate isomerase.
catalyzes the reversible isomerization of isopentenyl diphosphate (IPP) to dimethylallyl diphosphate (DMAPP), a key step in the biosynthesis of isoprenoids.
This gene is a mutant of idi from Escherichia coli JM109. idi include SpeⅠ site, so this point was muted to create Biobrick parts.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]