Difference between revisions of "Part:BBa K3317001"

Revision as of 22:50, 21 October 2019

Vanillate O-demethylase (LigM gene) from Sphingobium sp. SYK-6 with the pduP signal peptide

Vanillate O-demethylase (LigM)(BBa_K3317000), was modified with a signal peptide at its C-terminal to be introduced into bacterial microcompatments (BMCs) This signal peptide corresponds to the first 19 amino acids of the psuP enzyme together with a flexible linker G42 (Wagner, H. J et al. 2017)

This dimeric enzyme is tetrahydrofolate-dependent.

File:T--UANL--circuit--2 plasmid.png

alt text

Wild type enzyme information: E. C. 2.1.1.341

Km 0.078

Kcat 7.1292

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 369
Illegal NgoMIV site found at 1150
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1372

@@ Line 6: / Line 6: @@
 This dimeric enzyme is tetrahydrofolate-dependent.
-[[File:T--UANL--LigM.jpeg|200px|thumb|left|alt text]]
+[[File:T--UANL--circuit--2_plasmid.png|200px|thumb|left|alt text]]
 Wild type enzyme information:
 E. C. 2.1.1.341