Difference between revisions of "Part:BBa K3267001"
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Transformed cells with this construct respond to AHL-dependent quorum sensing and GFP would be expressed by the activated transcription factor protein LuxR which binds to the bidirectional promoter region (pLuxR/pLuxI).
 
Transformed cells with this construct respond to AHL-dependent quorum sensing and GFP would be expressed by the activated transcription factor protein LuxR which binds to the bidirectional promoter region (pLuxR/pLuxI).
  
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===Biology===
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===Biology&Usage===
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The Receiver part contains Vibrio fischeri intrinsic the bidirectional promoter region of the LuxRS regulon. The high concentration of Acyl-homoserine lactose (AHL) binds to the LuxR protein to activate and it binds to pLuxR/pLuxS promoters. Instead of LuxS protein, we add super folder GFP in order to make this part as the reporter part of the whole system (Fig 1). The super folder GFP is followed by the PixCell degradation tag from the 2018 Imperial College team to analyze the reporter gene spatiotemporally.
+
This Receiver part contains the V. fischeri-intrinsic bidirectional promoter region, the LuxRS regulon. The high concentration of Acyl-homoserine lactose (AHL) binds to the LuxR protein to activate and it binds to pLuxR/pLuxS promoters. Instead of LuxS protein, we add super folder GFP in order to make this part as the reporter part of the whole system (Fig 1). The super folder GFP is followed by the PixCell degradation tag from the 2018 Imperial College team to analyze the reporter gene spatiotemporally.
  
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===Usage===
 
  
 
===Characterization===
 
===Characterization===
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Revision as of 22:42, 21 October 2019


Quorum-Sensing Receiver Construct

Transformed cells with this construct respond to AHL-dependent quorum sensing and GFP would be expressed by the activated transcription factor protein LuxR which binds to the bidirectional promoter region (pLuxR/pLuxI).

Biology&Usage

This Receiver part contains the V. fischeri-intrinsic bidirectional promoter region, the LuxRS regulon. The high concentration of Acyl-homoserine lactose (AHL) binds to the LuxR protein to activate and it binds to pLuxR/pLuxS promoters. Instead of LuxS protein, we add super folder GFP in order to make this part as the reporter part of the whole system (Fig 1). The super folder GFP is followed by the PixCell degradation tag from the 2018 Imperial College team to analyze the reporter gene spatiotemporally.


Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1015
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1661