Difference between revisions of "Part:BBa K3117020:Design"

Revision as of 22:31, 21 October 2019

scFv VH against CD3 codon optimized for CHO expression

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 142
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This part was codon optimized for expression in mammalian cells.

Source

The sequence of this part was provided by Prof. Matthias Peipp from the Christian-Albrechts-Universität of Kiel.

References

[1]: Carreras-Sangrà, Nelson; Tomé-Amat, Jaime; García-Ortega, Lucía; Batt, Carl A.; Oñaderra, Mercedes; Martínez-del-Pozo, Alvaro et al. (2012): Production and characterization of a colon cancer-specific immunotoxin based on the fungal ribotoxin α-sarcin. In: Protein engineering, design & selection : PEDS 25 (8), S. 425–435. DOI: 10.1093/protein/gzs032.

[2]: Chetty, R.; Gatter, K. (1994): CD3: structure, function, and role of immunostaining in clinical practice. In: The Journal of pathology 173 (4), S. 303–307. DOI: 10.1002/path.1711730404.

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	[1]: Carreras-Sangrà, Nelson; Tomé-Amat, Jaime; García-Ortega, Lucía; Batt, Carl A.; Oñaderra, Mercedes; Martínez-del-Pozo, Alvaro et al. (2012): Production and characterization of a colon cancer-specific immunotoxin based on the fungal ribotoxin α-sarcin. In: Protein engineering, design & selection : PEDS 25 (8), S. 425–435. DOI: 10.1093/protein/gzs032.		[1]: Carreras-Sangrà, Nelson; Tomé-Amat, Jaime; García-Ortega, Lucía; Batt, Carl A.; Oñaderra, Mercedes; Martínez-del-Pozo, Alvaro et al. (2012): Production and characterization of a colon cancer-specific immunotoxin based on the fungal ribotoxin α-sarcin. In: Protein engineering, design & selection : PEDS 25 (8), S. 425–435. DOI: 10.1093/protein/gzs032.
		+
		+	[2]: Chetty, R.; Gatter, K. (1994): CD3: structure, function, and role of immunostaining in clinical practice. In: The Journal of pathology 173 (4), S. 303–307. DOI: 10.1002/path.1711730404.