Difference between revisions of "Part:BBa K1741014:Experience"
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'''Figure 2:''' BL21 and DH5a(MG1655) RFU/OD Measurements
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'''Figure 6:''' pZe proD sfGFP OD Measurements
 
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'''Figure 7:''' pZa proD sfGFP OD Measurements
 
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'''Figure 8:''' pZs proD sfGFP OD Measurements
 
 
===User Reviews===
 
===User Reviews===
 
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Revision as of 21:43, 21 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1741014

Bilkent-UNAMBG 2019 iGEM Team

We characterized the ProD-sfGFP part (Bba_K1741014) by cloning this part into pZA, pZE and pZS backbone. We obtained the fluorescence data by measuring the fluorescence level for each 30 minutes for 6 hours. Also, we showed the growth curve of the cell containing the different origin plasmids with the different strains. We did not observe any significant differences between different strains within the context of fluorescence and OD. RFU/OD graphics illustrates the relative fluorescence units per optical density which depicts the fluoresce which are expressed in a certain unit relatively to cell growth.


T--Bilkent-UNAMBG--WT.png


Figure 1:BL21 and DH5a(MG1655) OD Measurements



T--Bilkent-UNAMBG--PZEpro.png


Figure 3: pZe proD sfGFP RFU/OD Measurements

T--Bilkent-UNAMBG--pzaProD.png


Figure 4: pZa proD sfGFP RFU/OD Measurements


T--Bilkent-UNAMBG--pzsProD.png


Figure 5: pZs proD sfGFP RFU/OD Measurements


User Reviews

UNIQe26db43ae427e751-partinfo-00000000-QINU UNIQe26db43ae427e751-partinfo-00000001-QINU