Difference between revisions of "Part:BBa K3257011"
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Efficient ribosome binding site from bacteriophage T7 gene 10. | Efficient ribosome binding site from bacteriophage T7 gene 10. | ||
| − | + | ===Biology=== | |
We measured the relative fluorescence intensity of EGFP expressed by T7 promoter (BBa_K3257000) and this RBS to prove that this RBS can work. | We measured the relative fluorescence intensity of EGFP expressed by T7 promoter (BBa_K3257000) and this RBS to prove that this RBS can work. | ||
[[File:Fluorescence of EGFP.jpeg|center|400px|thumb|'''Figure 1. The relative fluorescence intensity of EGFP with T7 promoter measured by 96-well plate reader''' EGFP represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.]] | [[File:Fluorescence of EGFP.jpeg|center|400px|thumb|'''Figure 1. The relative fluorescence intensity of EGFP with T7 promoter measured by 96-well plate reader''' EGFP represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.]] | ||
| − | + | ===Used by 2021 Fudan=== | |
| − | === | + | Please check our parts at https://2021.igem.org/Team:Fudan/Parts |
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Revision as of 02:57, 21 October 2021
T7 RBS
Efficient ribosome binding site from bacteriophage T7 gene 10.
Biology
We measured the relative fluorescence intensity of EGFP expressed by T7 promoter (BBa_K3257000) and this RBS to prove that this RBS can work.
Figure 1. The relative fluorescence intensity of EGFP with T7 promoter measured by 96-well plate reader EGFP represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding EGFP protein. The Negative represents the relative fluorescence intensity of BL21(DE3) transformed with plasmid encoding nonsense mutated EGFP protein. Significant difference between them shows that EGFP can be expressed and function well inside E.coli cells.
Used by 2021 Fudan
Please check our parts at https://2021.igem.org/Team:Fudan/Parts
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]