Difference between revisions of "Part:BBa K3122000"
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<partinfo>BBa_K2656000 short</partinfo>
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<partinfo>BBa K3122000 short</partinfo>
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[[File:T--Valencia_UPV--im11UPV2018.png|250px|thumb|centro|alt=domestication.|Figure 1. DNA basic parts domestication. First construction refers to promoter GB domestication. ]]Part BBa_K2656000 is the constitutive promoter derived from the T7 bacteriophage that allows high expression of proteins in the presence of the T7 DNA polymerase [https://parts.igem.org/Part:BBa_I719005  BBa_I719005 ] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>.<!-- Add more about the biology of this part here
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E. coli LamB is one of the best characterized and most flexible heterologous peptide carriers. This is due to its extracelular domains, consisting mainly of long loops. One of these loops can tolerate insertions of rather long sequences, between amino acid residues 153 and 154 (Sousa, 1996).  
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===Usage and Biology===<!-- -->
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Our group have adapted this functional display system to type IIS enzymes grammar, and called it AEGIS's lamB display system for Gram-negative bacteria. This part was designed to use the outer membrane protein lamB of E. coli K12 strain to express any tag or short sequence to the extracellular medium on its specific permissive loop. The advantages of this approach are notable: not only can it be used for bacteria with cell wall ensuring its right functioning, but also it is really simple and easy to use following MoClo standard.
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To see the design of this part go to the Design tab.
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<h2> Biology </h2>
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LamB is maltoporin precursor of Gram-negative bacteria. This outer membrane proteins family is involved in transport of maltodextrins across the outer membrane. Besides, LamB is the receptor of several different bacteriophagues as lambda fague.  
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Gene sequence of LamB [Escherichia coli str. K-12 substr. MG1655] was obtained from EcoGene:EG10528.
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<h2> Characterization </h2>
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[[File:T--MADRID_UCM--wp-content~uploads~2019~10~nada_2.png|900px|thumb|none|alt=sfGFP spectra.|Figure 2. sfGFP emission and excitation spectra]]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2656000 SequenceAndFeatures</partinfo><!-- Uncomment this to enable Functional Parameter display
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<partinfo>BBa_K2656000 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2656000 parameters</partinfo>
 
<partinfo>BBa_K2656000 parameters</partinfo>
 
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Revision as of 21:05, 21 October 2019

No part name specified with partinfo tag. E. coli LamB is one of the best characterized and most flexible heterologous peptide carriers. This is due to its extracelular domains, consisting mainly of long loops. One of these loops can tolerate insertions of rather long sequences, between amino acid residues 153 and 154 (Sousa, 1996).

Our group have adapted this functional display system to type IIS enzymes grammar, and called it AEGIS's lamB display system for Gram-negative bacteria. This part was designed to use the outer membrane protein lamB of E. coli K12 strain to express any tag or short sequence to the extracellular medium on its specific permissive loop. The advantages of this approach are notable: not only can it be used for bacteria with cell wall ensuring its right functioning, but also it is really simple and easy to use following MoClo standard.

To see the design of this part go to the Design tab.

Biology

LamB is maltoporin precursor of Gram-negative bacteria. This outer membrane proteins family is involved in transport of maltodextrins across the outer membrane. Besides, LamB is the receptor of several different bacteriophagues as lambda fague. Gene sequence of LamB [Escherichia coli str. K-12 substr. MG1655] was obtained from EcoGene:EG10528.

Characterization

File:T--MADRID UCM--wp-content~uploads~2019~10~nada 2.png
Figure 2. sfGFP emission and excitation spectra

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]