Difference between revisions of "Part:BBa K3122000"

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Introducción
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__NOTOC__
 
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<partinfo>BBa_K2656000 short</partinfo>
Presentar el sistema
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[[File:T--Valencia_UPV--im11UPV2018.png|250px|thumb|centro|alt=domestication.|Figure 1. DNA basic parts domestication. First construction refers to promoter GB domestication. ]]Part BBa_K2656000 is the constitutive promoter derived from the T7 bacteriophage that allows high expression of proteins in the presence of the T7 DNA polymerase [https://parts.igem.org/Part:BBa_I719005  BBa_I719005 ] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the <html><a href="https://static.igem.org/mediawiki/parts/b/bc/T--Valencia_UPV--protocols.pdf#page=27"> Golden Gate assembly protocol </a></html>.<!-- Add more about the biology of this part here
 
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===Usage and Biology===<!-- -->
<h2>Biology</h2>
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<span class='h3bb'>Sequence and Features</span>
<h3>Titulo pequeño</h3>
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<partinfo>BBa_K2656000 SequenceAndFeatures</partinfo><!-- Uncomment this to enable Functional Parameter display
Viene de e.coli, es el receptor de fago lambda
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===Functional Parameters===
 
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<partinfo>BBa_K2656000 parameters</partinfo>
<h2>Characterization</h2>
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Foto placas/grafica
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[[File:/wiki/images/d/d3/T--MADRID_UCM--wp-content~uploads~2039~10~nada_2.png|caption]]
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<h2> How to use it</h2>
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<h2>References</h2>
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<h2>Judging</h2>
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Revision as of 20:52, 21 October 2019

T7 phage strong promoter

domestication.
Figure 1. DNA basic parts domestication. First construction refers to promoter GB domestication.
Part BBa_K2656000 is the constitutive promoter derived from the T7 bacteriophage that allows high expression of proteins in the presence of the T7 DNA polymerase BBa_I719005 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid 3.0] assembly methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the Golden Gate assembly protocol .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]