Difference between revisions of "Part:BBa K2992031"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This parts entry represents the RBS for the <i>ctfA</i> gene in <i>C. acetobutylicum</i>. The CtfAB complex is central to the production of biotechnologically relevant solvents in <i>C. acetobutylicum</i> such as acetone and butanol. The complex catalyses the conversion of acetoacetyl-CoA to acetoacetate concomitant with the transfer of CoA to acetate and butyrate. The CtfAB complex is a core component of our acetone production pathway to validate the use of volatile reporters to predict botulinum toxin production following food manufacturing processes. We have tested both <i>C. acetobutylicum</i> and <i>C. botulinum</i>-derived <i> | + | This parts entry represents the RBS for the <i>ctfA</i> gene in <i>C. acetobutylicum</i>. The CtfAB complex is central to the production of biotechnologically relevant solvents in <i>C. acetobutylicum</i> such as acetone and butanol. The complex catalyses the conversion of acetoacetyl-CoA to acetoacetate concomitant with the transfer of CoA to acetate and butyrate. The CtfAB complex is a core component of our acetone production pathway to validate the use of volatile reporters to predict botulinum toxin production following food manufacturing processes. We have tested both <i>C. acetobutylicum</i> and <i>C. botulinum</i>-derived <i>ctfA</i> and <i>ctfB</i>. <br><br> |
===Characterisation=== | ===Characterisation=== | ||
Revision as of 20:57, 21 October 2019
RBS region for the ctfA gene from C. acetobutylicum
RBS for the ctfA gene from C. acetobutylicum
Usage and Biology
This parts entry represents the RBS for the ctfA gene in C. acetobutylicum. The CtfAB complex is central to the production of biotechnologically relevant solvents in C. acetobutylicum such as acetone and butanol. The complex catalyses the conversion of acetoacetyl-CoA to acetoacetate concomitant with the transfer of CoA to acetate and butyrate. The CtfAB complex is a core component of our acetone production pathway to validate the use of volatile reporters to predict botulinum toxin production following food manufacturing processes. We have tested both C. acetobutylicum and C. botulinum-derived ctfA and ctfB.
Characterisation
This part is the ribosome binding site for the translation of the ctfA gene from C. acetobutylicum . This was integrated in to the larger composite parts: BBa_K2992034, BBa_K2992035 and BBa_K2992036. See results pagefor more
===Sequence and Features===
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Cornillot, E., Croux, C. and Soucaille, P. (1997). Physical and genetic map of the Clostridium acetobutylicum ATCC 824 chromosome. Journal of Bacteriology, 179(23), pp.7426-7434.