Difference between revisions of "Part:BBa K3110048:Design"
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Codon optimisation was done using Twist Codon optimisation too to make it synthesis compatible in E.coli. The FLAG-tag at the end can be used to detect IL12 produced by E.coli. | Codon optimisation was done using Twist Codon optimisation too to make it synthesis compatible in E.coli. The FLAG-tag at the end can be used to detect IL12 produced by E.coli. | ||
| + | Also took help from the sequence designed by UNICAMP EMSE Brazil team (iGEM2011) - [[Part:BBa_K554005]] | ||
===Source=== | ===Source=== | ||
Revision as of 20:12, 21 October 2019
IL12 FLAG-tag (Codon Optimized)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimisation was done using Twist Codon optimisation too to make it synthesis compatible in E.coli. The FLAG-tag at the end can be used to detect IL12 produced by E.coli. Also took help from the sequence designed by UNICAMP EMSE Brazil team (iGEM2011) - Part:BBa_K554005
Source
Sequence was synthesized by Twist Bioscience.
References
- K. R. Morris et al, Expression of biologically active recombinant porcine interleukin-12 from Escherichia coli, Veterinary Immunology and Immunopathology, 2008, 126 Link to PubMed
- Sperandio V, Torres AG, Kaper JB (2002) Quorum sensing Escherichia coli regulators B and C (QseBC): a novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli. Mol Microbiol 43: 809–821
- Part:BBa_K554005 designed by UNICAMP EMSE Brazil team