Difference between revisions of "Part:BBa K136015:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | pBad/araC was digested by SpeI -PstI (insert) and cloned into the psb2k3 that | + | pBad/araC was digested by SpeI -PstI (insert) and cloned into the psb2k3 that contains the gfp tripart . |
===Source=== | ===Source=== | ||
Revision as of 14:47, 26 October 2008
pBad/araC - GFP tripart
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1883
Illegal SapI site found at 961
Design Notes
pBad/araC was digested by SpeI -PstI (insert) and cloned into the psb2k3 that contains the gfp tripart .
Source
flgA promoter comes from E. coli K12 strain MG1655. gfp tripart comes from the registry.