Difference between revisions of "Part:BBa K2992031"

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===Characterisation===
 
===Characterisation===
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This part is the ribosome binding site for the translation of the <i> ctfA </i> gene from <i> C. acetobutylicum </i>. This was integrated in to the larger composite part: [https://parts.igem.org/Part:BBa_K2992035 BBa_K2992035]. See [https://2019.igem.org/Team:Nottingham/Results results page]for more <br> <span class='h3bb'>===Sequence and Features===</span>
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This part is the ribosome binding site for the translation of the <i> ctfA </i> gene from <i> C. acetobutylicum </i>. This was integrated in to the larger composite parts: [https://parts.igem.org/Part:BBa_K2992034 BBa_K2992034], [https://parts.igem.org/Part:BBa_K2992035 BBa_K2992035] and [https://parts.igem.org/Part:BBa_K2992036 BBa_K2992036]. See [https://2019.igem.org/Team:Nottingham/Results results page]for more <br> <span class='h3bb'>===Sequence and Features===</span>
 
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Revision as of 20:21, 21 October 2019


RBS region for the ctfA gene from C. acetobutylicum

RBS for the ctfA gene from C. acetobutylicum


Usage and Biology

This parts entry represents the RBS for the ctfA gene in C. acetobutylicum. The CtfAB complex is central to the production of biotechnologically relevant solvents in C. acetobutylicum such as acetone and butanol. The complex catalyses the conversion of acetoacetyl-CoA to acetoacetate concomitant with the transfer of CoA to acetate and butyrate. The CtfAB complex is a core component of our acetone production pathway to validate the use of volatile reporters to predict botulinum toxin production following food manufacturing processes. We have tested both C. acetobutylicum and C. botulinum-derived ctfAB.

Characterisation

This part is the ribosome binding site for the translation of the ctfA gene from C. acetobutylicum . This was integrated in to the larger composite parts: BBa_K2992034, BBa_K2992035 and BBa_K2992036. See results pagefor more
===Sequence and Features===

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Cornillot, E., Croux, C. and Soucaille, P. (1997). Physical and genetic map of the Clostridium acetobutylicum ATCC 824 chromosome. Journal of Bacteriology, 179(23), pp.7426-7434.