Difference between revisions of "Part:BBa K3183002"

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The lactate dehydrogenase (ldhL) promoter is a constitutive promoter that has been used in <i> Lactobacillus reuteri </i> in our composite part BBa_K3183100. This is a constitutive promoter from the lactate dehydrogenase (ldhL) promoter from <i>Lactobacillus acidophilus</i> (Kim et al., 1991). It was characterised in six strains of <i>Lactobacillus reuteri</i> and <i>Lactococcus lactis </i>spp.<i> cremoris</i> MG1363 by <a href="https://academic.oup.com/femsle/article/308/1/8/513999">Lizier et al, 2010.</a>
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The lactate dehydrogenase (ldhL) promoter is a constitutive promoter that has been used in <i> Lactobacillus reuteri </i> in our composite part BBa_K3183100. This is a constitutive promoter from the lactate dehydrogenase (ldhL) promoter from <i>Lactobacillus acidophilus</i><sup>1</sup>. It has been subsequently characterised in six strains of <i>Lactobacillus reuteri</i> and <i>Lactococcus lactis </i>spp.<i> cremoris</i> MG1363<sup>2</sup>.
 
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         <!--img src="https://static.igem.org/mediawiki/2018/5/58/T--Oxford--promoters.png"alt=width="250" height="190" style="float:center">
 
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===References===
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1. Kim, S F et al. “Cloning and nucleotide sequence of the Lactobacillus casei lactate dehydrogenase gene.” Applied and environmental microbiology vol. 57,8 (1991): 2413-7.
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2. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.

Revision as of 19:56, 21 October 2019


Lactate Dehydrogenase Constitutive Promoter for Lactobacillus sp.

P-slp is a constitutive promoter which can be used in Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species.

The lactate dehydrogenase (ldhL) promoter is a constitutive promoter that has been used in Lactobacillus reuteri in our composite part BBa_K3183100. This is a constitutive promoter from the lactate dehydrogenase (ldhL) promoter from Lactobacillus acidophilus1. It has been subsequently characterised in six strains of Lactobacillus reuteri and Lactococcus lactis spp. cremoris MG13632.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




Characterisation

The part was characterised in the composite part BBa_K3183101