Difference between revisions of "Part:BBa K3002218"

 
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<partinfo>BBa_K3002218 short</partinfo>
 
<partinfo>BBa_K3002218 short</partinfo>
  
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This composite part contains a hygromycin resistance (BBa_K3002119) and the MHETase with the secretion signal cCA (BBa_K3002114) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
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<html>
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This composite part contains a hygromycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002119">BBa_K3002119</a>) and the MHETase with the secretion signal cCA (<a href="https://parts.igem.org/Part:BBa_K3002114">BBa_K3002114</a>) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.
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</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:46, 22 October 2019


L2 Hygromycin resistance + L1_connector_Pos2 + ccA_MHETase_SP20HA

This composite part contains a hygromycin resistance (BBa_K3002119) and the MHETase with the secretion signal cCA (BBa_K3002114) fused with an SP20HA-tag for easy detection via HA-antibody and enhanced secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3126
    Illegal PstI site found at 3450
    Illegal PstI site found at 3793
    Illegal PstI site found at 4603
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2048
    Illegal PstI site found at 3126
    Illegal PstI site found at 3450
    Illegal PstI site found at 3793
    Illegal PstI site found at 4603
    Illegal NotI site found at 3461
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4371
    Illegal BamHI site found at 1408
    Illegal XhoI site found at 250
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3126
    Illegal PstI site found at 3450
    Illegal PstI site found at 3793
    Illegal PstI site found at 4603
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3126
    Illegal PstI site found at 3450
    Illegal PstI site found at 3793
    Illegal PstI site found at 4603
    Illegal NgoMIV site found at 773
    Illegal NgoMIV site found at 955
    Illegal NgoMIV site found at 1326
    Illegal NgoMIV site found at 3059
    Illegal NgoMIV site found at 3520
    Illegal NgoMIV site found at 3550
    Illegal NgoMIV site found at 3865
  • 1000
    COMPATIBLE WITH RFC[1000]