Difference between revisions of "Part:BBa K1021007"
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===Characterization=== | ===Characterization=== | ||
<b>DTU-Denmark 2019 characterization</b> | <b>DTU-Denmark 2019 characterization</b> | ||
| + | The DTU-Denmark 2019 team has characterized the PtrpC promoter in <i>Aspergillus niger</i>. Our characterization experiments were done in two different scales: 1.5 ml wells in a microbioreactor (BioLector), and 10 mL cultures in specialized 50 mL falcon tubes. | ||
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Revision as of 19:51, 21 October 2019
PtrpC
PtrpC is a strong constitutive promoter from Aspergillus nidulans's tryptophan biosynthesis pathways. It was isolated using a fungal transformation vector and constructed into the pSB1C3 backbone for continuous homologous and heterologous gene expression in fungal chassis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
To test the activity of the trpC promoter, a composite part was created with GFP downstream (BBa_K1021023). This part was transformed into the ascomycete fungi Cochliobolus heterostrophus. Results indicated that the trpC promoter provided GFP expression within this fungal chassis.
Characterization
DTU-Denmark 2019 characterization The DTU-Denmark 2019 team has characterized the PtrpC promoter in Aspergillus niger. Our characterization experiments were done in two different scales: 1.5 ml wells in a microbioreactor (BioLector), and 10 mL cultures in specialized 50 mL falcon tubes.