Difference between revisions of "Part:BBa K3304105"

 
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This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG (part: ) wi initiates the strand displacement cascades. Other auxiliary strands for this gate are BTD (part: ) and NTB (part: ) who conclude the reactions and facilitate their trajectory to the desired direction. The output of this gate is TBB (part ) which constitutes the input for the downstream Join Gate (part ). The part comes with two PvuII cut sites and three Nt.BstNBI nicking sites that facilitate the extraction of the gate from plasmid DNA and prepare the double-stranded block for strand displacement reactions.
 
This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG (part: ) wi initiates the strand displacement cascades. Other auxiliary strands for this gate are BTD (part: ) and NTB (part: ) who conclude the reactions and facilitate their trajectory to the desired direction. The output of this gate is TBB (part ) which constitutes the input for the downstream Join Gate (part ). The part comes with two PvuII cut sites and three Nt.BstNBI nicking sites that facilitate the extraction of the gate from plasmid DNA and prepare the double-stranded block for strand displacement reactions.
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https://2019.igem.org/File:T--Thessaloniki--results-f1.png
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 02:14, 22 October 2019


Input Gate with ELK1 sequence

This is the Input Gate for our Chemical Reaction Network. The oligo input is DTG (part: ) wi initiates the strand displacement cascades. Other auxiliary strands for this gate are BTD (part: ) and NTB (part: ) who conclude the reactions and facilitate their trajectory to the desired direction. The output of this gate is TBB (part ) which constitutes the input for the downstream Join Gate (part ). The part comes with two PvuII cut sites and three Nt.BstNBI nicking sites that facilitate the extraction of the gate from plasmid DNA and prepare the double-stranded block for strand displacement reactions. https://2019.igem.org/File:T--Thessaloniki--results-f1.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]