Difference between revisions of "Part:BBa K3002223"

Revision as of 01:48, 22 October 2019

L2 spectinomycin resistance + GLE_Mut-PETase_SP20His

This composite part contains a spectinomycin resistance (BBa_K3002102) and the mutant PETase with the secretion signal GLE (BBa_K3002122) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion. Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2401
Illegal PstI site found at 3503
Illegal PstI site found at 4476
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2401
Illegal NheI site found at 2665
Illegal PstI site found at 3503
Illegal PstI site found at 4476
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2401
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2401
Illegal PstI site found at 3503
Illegal PstI site found at 4476
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2401
Illegal PstI site found at 3503
Illegal PstI site found at 4476
Illegal NgoMIV site found at 1401
Illegal NgoMIV site found at 1584
Illegal NgoMIV site found at 1694
Illegal NgoMIV site found at 3238
Illegal NgoMIV site found at 3265
Illegal NgoMIV site found at 4967
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3002223 short</partinfo>
-This composite part contains a spectinomycin resistance (BBa_K3002102) and the mutant PETase with the secretion signal GLE (BBa_K3002122) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion.
+<html>
+This composite part contains a spectinomycin resistance (<a href="https://parts.igem.org/Part:BBa_K3002102">BBa_K3002102</a>) and the mutant PETase with the secretion signal GLE (<a href="https://parts.igem.org/Part:BBa_K3002122">BBa_K3002122</a>) fused with an SP20His-tag for easy purification via Ni-NTA column and enhanced secretion.
+</html>
 <!-- Add more about the biology of this part here
 ===Usage and Biology===