Difference between revisions of "Part:BBa K3179004"
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[[File:T-SYSU-CHINA-pTRE-E.jpg|500px|thumb|left]]
 
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Since the gene product of the E1 can initiate the gene replication of adenovirus and it’s not been reported that it has any other function in eukaryotic cells, SYSU-CHINA decided to transfection pTRE-E1A, which is a plasmid containing E1A gene regulated by pTRE, with replication-defective adenovirus, which lacks the E1 gene, into HeLa cell. The cell experiment showed that replication-defective adenovirus can’t replicate and lyse cells. However, if there is E1A existed, the virus can be autonomously replicable and lyse cells. As we see, higher concentration of pTRE-E1A can make better effect.
 
Since the gene product of the E1 can initiate the gene replication of adenovirus and it’s not been reported that it has any other function in eukaryotic cells, SYSU-CHINA decided to transfection pTRE-E1A, which is a plasmid containing E1A gene regulated by pTRE, with replication-defective adenovirus, which lacks the E1 gene, into HeLa cell. The cell experiment showed that replication-defective adenovirus can’t replicate and lyse cells. However, if there is E1A existed, the virus can be autonomously replicable and lyse cells. As we see, higher concentration of pTRE-E1A can make better effect.
  
 
The cell viability assay is shown below. It can be easily seen that the cell viabilities in experimental group are lower than the control ones. Although the effect is not as good as we thought, it still means that it can function.
 
The cell viability assay is shown below. It can be easily seen that the cell viabilities in experimental group are lower than the control ones. Although the effect is not as good as we thought, it still means that it can function.
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[[File:T-SYSU-CHINA-pTRE-E1A.png|500px|thumb|left]]
 
[[File:T-SYSU-CHINA-pTRE-E1A.png|500px|thumb|left]]
  

Revision as of 18:41, 21 October 2019


2Kt-E1A

T-SYSU-CHINA-pTRE-E.jpg


Since the gene product of the E1 can initiate the gene replication of adenovirus and it’s not been reported that it has any other function in eukaryotic cells, SYSU-CHINA decided to transfection pTRE-E1A, which is a plasmid containing E1A gene regulated by pTRE, with replication-defective adenovirus, which lacks the E1 gene, into HeLa cell. The cell experiment showed that replication-defective adenovirus can’t replicate and lyse cells. However, if there is E1A existed, the virus can be autonomously replicable and lyse cells. As we see, higher concentration of pTRE-E1A can make better effect.

The cell viability assay is shown below. It can be easily seen that the cell viabilities in experimental group are lower than the control ones. Although the effect is not as good as we thought, it still means that it can function.

T-SYSU-CHINA-pTRE-E1A.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 859
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 63
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 859
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 859
    Illegal NgoMIV site found at 333
    Illegal AgeI site found at 69
  • 1000
    COMPATIBLE WITH RFC[1000]