Difference between revisions of "Part:BBa J10054:Experience"
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===User Reviews=== | ===User Reviews=== | ||
= Lambert_GA 2019 Characterization = | = Lambert_GA 2019 Characterization = | ||
| − | ==Characterization of medium promoter (BBa_J23106) and | + | ==Characterization of medium promoter (BBa_J23106) and medium RBS (BBa_B0032)== |
Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10054 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10053 and BBa_J10055, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths. | Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10054 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10053 and BBa_J10055, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths. | ||
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<center> [[File:T--Lambert GA--tuning5a.png|800px]] </center> | <center> [[File:T--Lambert GA--tuning5a.png|800px]] </center> | ||
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| − | <center> <i>Figure 1: The highlighted points in yellow represent BBa_J10054 (medium promoter/ | + | <center> <i>Figure 1: The highlighted points in yellow represent BBa_J10054 (medium promoter/ medium RBS) compared to BBa_J10053 (medium promoter/ weak RBS) and BBa_J10055 (medium promoter/ strong RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter.</i> </center> |
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<center> [[File:T--Lambert GA--tuning5b.png|800px]] </center> | <center> [[File:T--Lambert GA--tuning5b.png|800px]] </center> | ||
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| − | <center> <i>Figure 2: The highlighted points represent the BBa_J10054 composite part (medium promoter/ | + | <center> <i>Figure 2: The highlighted points represent the BBa_J10054 composite part (medium promoter/ medium RBS). |
As the promoter strength increases, the expression increases as well.</i> | As the promoter strength increases, the expression increases as well.</i> | ||
<br> | <br> | ||
Revision as of 18:42, 21 October 2019
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J10054
Lambert_GA 2019 used this part in experiments to characterize the effect of different strengths of promoters and ribosomal binding sites on expression. The data obtained can aid in the design of constructs by giving information about controlling expression.
User Reviews
Lambert_GA 2019 Characterization
Characterization of medium promoter (BBa_J23106) and medium RBS (BBa_B0032)
Differences in promoter strength had more effect on enzymatic expression compared to differences in RBS strengths as evidenced in the graphs below. Lambert_GA 2019 had originally thought that BBa_J10054 would result in a moderate amount of expression out of the tested combinations, but this part, along with BBa_J10053 and BBa_J10055, all shared moderate expression out of the combinations. This is due to the translation rate not being an important factor in the determination of expression of ONP; however, the promoter strength is a significant factor. In other words, expression values are significantly different with different promoters but not with different RBS strengths.
As the promoter strength increases, the expression increases as well.
The graphs above show that RBS strength has a negligible effect on expression, whereas increased promoter strength increases expression.
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