Difference between revisions of "Part:BBa K2940007"
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<partinfo>BBa_K2940007 short</partinfo>
 
<partinfo>BBa_K2940007 short</partinfo>
  
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This part is designed as the control group to check whether the original DyP can be expressed and degrade dyes. So the coding sequence was refrenenced from NCBI () without any modification. The strong constitutive promoter and RBS combination were introduced to make the DNA construct. It was cloned into pSB1C3 using classcial cloning and was verified using ABTS assay.
 
  
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Dye-degrading peroxidase is from Bacillus subtilis.
 
  
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===Usage and Biology===
 
===Usage and Biology===
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Dye-degrading peroxidase (DyP) is from Bacillus subtilis. This part is designed as the control group to check whether the original DyP can be expressed and degrade dyes. So the coding sequence was referenced from NCBI (NC_000964.3) without any modification. (NC_000964.3)  It was cloned into pSB1C3 using classical cloning and was verified using SDS-PAGE analysis and ABTS assay.
  
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K2940007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2940007 SequenceAndFeatures</partinfo>
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Revision as of 18:17, 21 October 2019


Secreted dye-degrading peroxidase（DyP)


Usage and Biology

Dye-degrading peroxidase (DyP) is from Bacillus subtilis. This part is designed as the control group to check whether the original DyP can be expressed and degrade dyes. So the coding sequence was referenced from NCBI (NC_000964.3) without any modification. (NC_000964.3) It was cloned into pSB1C3 using classical cloning and was verified using SDS-PAGE analysis and ABTS assay.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 10
    Illegal NheI site found at 33
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 155
    Illegal BsaI site found at 164
    Illegal BsaI site found at 1130
    Illegal SapI site found at 560
    Illegal SapI site found at 918
    Illegal SapI.rc site found at 1074