Toxicity test with TPA, EG and EtOH. Growth of Chlamydomonas strain UVM4 was tested in TAP medium containing 0 mM (control), 5 mM and 10 mM terephtalic acid (TPA), ethylene glycol (EG) and ethanol (EtOH). The pH in the medium containing TPA was adjusted to pH 7 with potassium acetate. (a) UVM4 cells were inoculated in 20 mL with 2*105 cells/mL. Growth was measured by counting cells for 5 days. Error bars represent standard error of three biological replicates. (b) Spot test analysis with the same concentrations of EG, EtOH and TPA as used in (a). Note that the UVM4 strain lacks a cell wall and that cells die if they are too diluted.