Difference between revisions of "Part:BBa J10058:Experience"
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===Applications of BBa_J10058===
 
===Applications of BBa_J10058===
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Lambert 2019 used this part in experiments to model the effect of different promoter strengths and ribosomal binding strengths to develop a model to aid in controlling expression in constructs.
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Lambert_GA 2019 used this part in experiments to characterize the effect of different strengths of promoters and ribosomal binding sites on expression. The data obtained can aid in the design of constructs by giving information about controlling expression.  
  
 
===User Reviews===
 
===User Reviews===
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= Lambert_GA 2019 Characterization =
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==Characterization of Strong Promoter (BBa_J23119) and Strong RBS (BBa_B0034)==
 
<center> [[File:T--Lambert GA--tuning9a.png|800px]]
 
<center> [[File:T--Lambert GA--tuning9a.png|800px]]
 
<p> <i> Figure 1: The highlighted points in yellow represent BBa_J10058 (strong promoter/ strong RBS) compared to BBa_J10057 (strong promoter/ medium RBS) and BBa_J10056 (strong promoter/ weak RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter.
 
<p> <i> Figure 1: The highlighted points in yellow represent BBa_J10058 (strong promoter/ strong RBS) compared to BBa_J10057 (strong promoter/ medium RBS) and BBa_J10056 (strong promoter/ weak RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter.

Revision as of 18:46, 21 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J10058

Lambert_GA 2019 used this part in experiments to characterize the effect of different strengths of promoters and ribosomal binding sites on expression. The data obtained can aid in the design of constructs by giving information about controlling expression.

User Reviews

Lambert_GA 2019 Characterization

Characterization of Strong Promoter (BBa_J23119) and Strong RBS (BBa_B0034)

T--Lambert GA--tuning9a.png

Figure 1: The highlighted points in yellow represent BBa_J10058 (strong promoter/ strong RBS) compared to BBa_J10057 (strong promoter/ medium RBS) and BBa_J10056 (strong promoter/ weak RBS), both in light blue. The weak, medium, and strong RBS have similar expression levels when in combination with the weak promoter.


T--Lambert GA--tuning9b.png
Figure 2: The highlighted points represent the BBa_J10058 composite part (strong promoter/ strong RBS). As the promoter strength increases, the expression increases as well.
The graphs above show that RBS strength has a negligible effect on expression, whereas increased promoter strength increases expression.

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