Difference between revisions of "Part:BBa K3063901"
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| − | The part encodes artificial microRNA (amiRNA) that silences signal transducer and activator of transcription 3 (STAT3), a member of the STAT protein family for maintaining cancer stemness. <ref> | + | The part encodes artificial microRNA (amiRNA) that silences signal transducer and activator of transcription 3 (STAT3), a member of the STAT protein family for maintaining cancer stemness. <ref> Zhang, H. F., & Lai, R. (2014). STAT3 in cancer—friend or foe?. Cancers, 6(3), 1408-1440. </ref> STAT3-targeting amiRNA in the transformed salmonella, will form dsRNA upon binding to stemness STAT3 mRNA in tumour cells. Under the aid of this particular biobrick, RNA silencing and degradation of STAT3 mRNA in liver cancer stem cells can be achieved simultaneously. |
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| − | The BioBricks encodes STAT targeting amiRNA. With the help of enhanced | + | The BioBricks encodes STAT targeting amiRNA. With the help of enhanced synthetic inhibitory BIC/miR-155 RNA (eSIBR) vector, pri-miRNA hairpin as shown below is transcribed and later processed into mature miRNA in Salmonella Typhimurium that achieve STAT3 knockdown in target cancer cells. |
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Revision as of 17:41, 21 October 2019
eSIBR construct fragment for STAT3
The part encodes artificial microRNA (amiRNA) that silences signal transducer and activator of transcription 3 (STAT3), a member of the STAT protein family for maintaining cancer stemness. [1] STAT3-targeting amiRNA in the transformed salmonella, will form dsRNA upon binding to stemness STAT3 mRNA in tumour cells. Under the aid of this particular biobrick, RNA silencing and degradation of STAT3 mRNA in liver cancer stem cells can be achieved simultaneously.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 156
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Introduction
Biology
Part structure
The part for STAT3 silencing in cancer stem cells
BBa_K3063901 The BioBricks encodes STAT targeting amiRNA. With the help of enhanced synthetic inhibitory BIC/miR-155 RNA (eSIBR) vector, pri-miRNA hairpin as shown below is transcribed and later processed into mature miRNA in Salmonella Typhimurium that achieve STAT3 knockdown in target cancer cells.
Part Therapeutics
Recent findings have demonstrated that STAT3-mediated signaling is implicated in regulating the undifferentiated phenotype of stem cells and suppressing antitumor immunity, while in vitro silencing is capable of inducing G2/M arrest in a variety of cancer cell types [2] [3] [4] Hence, we postulated that STAT3-targeting amiRNA, carried by salmonella, can effectively silence STAT3 expression in liver stem cells, which in turns minimises the cancer stemness.
References
- ↑ Zhang, H. F., & Lai, R. (2014). STAT3 in cancer—friend or foe?. Cancers, 6(3), 1408-1440.
- ↑ Galoczova, M., Coates, P., & Vojtesek, B. (2018). STAT3, stem cells, cancer stem cells and p63. Cellular & molecular biology letters, 23(1), 12.
- ↑ Raz, R., Lee, C. K., Cannizzaro, L. A., d’Eustachio, P., & Levy, D. E. (1999). Essential role of STAT3 for embryonic stem cell pluripotency. Proceedings of the National Academy of Sciences, 96(6), 2846-2851.
- ↑ Li, J., Liu, Y. Y., Yang, X. F., Shen, D. F., Sun, H. Z., Huang, K. Q., & Zheng, H. C. (2018). Effects and mechanism of STAT3 silencing on the growth and apoptosis of colorectal cancer cells. Oncology letters, 16(5), 5575-5582.