Difference between revisions of "Part:BBa K2753045"

Group: BNDS_China iGEM 2019
Author: Wanji Li
Introduction & Design:

In order to determine the strength of this promoter at different temperature and glucose concentration, we constructed a plasmid with the expression of GFP as an indicator downstream the TEF1 promoter and measured its intensity of fluorescence. Additionally, we also constructed a plasmid with GFP only as a negative control.

FIGURE 1(A) the intensity of fluorescence under different concentration of glucose in 30 degree Celsius; (B) the intensity of fluorescence under different concentration of glucose in 32 degree Celsius; (C) the intensity of fluorescence under different concentration of glucose in 34 degree Celsius; (D) the intensity of fluorescence under different concentration of glucose in 37 degree Celsius