Difference between revisions of "Part:BBa K136051"

 
 
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__NOTOC__
 
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<partinfo>BBa_K136051 short</partinfo>
 
<partinfo>BBa_K136051 short</partinfo>
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It is activated by FlhD4C2 and maybe by FliA with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).  
 
It is activated by FlhD4C2 and maybe by FliA with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).  
  
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pFlhB is followed by EnvZ*
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In this construction, flhB promotor is followed by EnvZ*
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 10:21, 26 October 2008

flhB promoter + WT RBS - envZ*

Promoter of flhB gene from E. coli flagella

flhB is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ).

Its promoter is consitutively repressed.

It is activated by FlhD4C2 and maybe by FliA with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).

In this construction, flhB promotor is followed by EnvZ*

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 525
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 525
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 525
    Illegal BglII site found at 445
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 525
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 525
  • 1000
    COMPATIBLE WITH RFC[1000]