Difference between revisions of "Part:BBa K3225015"
Line 24: Line 24:
 
<body>
 
<body>
 
<img style="width:800px;"src="https://static.igem.org/mediawiki/parts/8/83/T--iBowu-China--Character-5015-fig1.jpeg">
 
<img style="width:800px;"src="https://static.igem.org/mediawiki/parts/8/83/T--iBowu-China--Character-5015-fig1.jpeg">
−
Figure 1. The detection limit and detection time of quorum sensing based testing strips.  
+
<p>Figure 1. The detection limit and detection time of quorum sensing based testing strips. </p>
  
  
 
</body>
 
</body>
 
</html>
 
</html>

Revision as of 16:50, 21 October 2019


J23101-LuxR-pLux-LacZ

This gene circuit is designed to detect N-(3-oxohexanoyl)-L-homoserine lactone (OHHL T--iBowu-China--DesignOHHL.png ，quorum sensing signal molecular of E.carotovora). It consists of LuxR(quorum sensing transcriptional factor, BBa_C0062) drove by a constitutive promoter J23101 and LacZ(BBa_I732017) as a repoter drove by the binding promoter of LuxR(pLux, BBa_R0062).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results

We placed the physical gene circuits to papers by freeze-drying technology. The report is LacZ which is β-galactosidase which can cleave the yellow substrate to purple. Among the series of AHL concentrations induction, it showed that the detection limit was lower than 1 nM. Moreover, there is a significant visible output with 10 nm AHL treatment (Fig. 1).

Figure 1. The detection limit and detection time of quorum sensing based testing strips.