Difference between revisions of "Part:BBa K2974700:Experience"
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Latest revision as of 16:50, 21 October 2019
Medium Promoter (BBa_J23106) Toehold GFP
The construction of the toehold was confirmed through successful transformation in Dh5a cells and sequencing results. The function of the toehold was confirmed through a dual plasmid transformation in BL21 E. coli with the complementary trigger. The dual plasmid transformation presented greater fluorescence measured in light intensity (lux) values in comparison to the single switch transformation, confirming the function of the toehold. Additionally, there were greater lux values associated with the single switch transformation and dual plasmid transformation of the strong promoter toehold when compared to the same toehold switch construct with a medium-strength promoter. The dual plasmid transformation would not succeed without the correct proof of concept toehold intermediate, so the function of this part (BBa_K2974700) has been validated through experimentation and sequencing. eGFP expression in the toehold system for promoters J23100 and J23106 was obtained through successful transformation and light intensity (lux) values were recorded.
Applications of BBa_K2974700
User Reviews
UNIQ1bf7cf9b8ecadd9d-partinfo-00000002-QINU UNIQ1bf7cf9b8ecadd9d-partinfo-00000003-QINU