Difference between revisions of "Part:BBa K3225015"
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<partinfo>BBa_K3225015 parameters</partinfo>
 
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==Results==
 
==Results==
 
We placed the physical gene circuits to papers by freeze-drying technology. The report is LacZ which is β-galactosidase which can cleave the yellow substrate to purple. Among the series of AHL concentrations induction, it showed that the detection limit was lower than 1 nM. Moreover, there is a significant visible output with 10 nm AHL treatment (Fig. 1).
 
We placed the physical gene circuits to papers by freeze-drying technology. The report is LacZ which is β-galactosidase which can cleave the yellow substrate to purple. Among the series of AHL concentrations induction, it showed that the detection limit was lower than 1 nM. Moreover, there is a significant visible output with 10 nm AHL treatment (Fig. 1).
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Figure 1. The detection limit and detection time of quorum sensing based testing strips.  
 
Figure 1. The detection limit and detection time of quorum sensing based testing strips.  
  

Revision as of 16:49, 21 October 2019


J23101-LuxR-pLux-LacZ

This gene circuit is designed to detect N-(3-oxohexanoyl)-L-homoserine lactone (OHHL T--iBowu-China--DesignOHHL.png ，quorum sensing signal molecular of E.carotovora). It consists of LuxR(quorum sensing transcriptional factor, BBa_C0062) drove by a constitutive promoter J23101 and LacZ(BBa_I732017) as a repoter drove by the binding promoter of LuxR(pLux, BBa_R0062).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results

We placed the physical gene circuits to papers by freeze-drying technology. The report is LacZ which is β-galactosidase which can cleave the yellow substrate to purple. Among the series of AHL concentrations induction, it showed that the detection limit was lower than 1 nM. Moreover, there is a significant visible output with 10 nm AHL treatment (Fig. 1). Figure 1. The detection limit and detection time of quorum sensing based testing strips.