Difference between revisions of "Part:BBa K3285700"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | This is an intermediate part which can be incorporated under a promoter. The pbrD protein is known to sequester lead ions in the cytoplasm of the bacterial cell. | ||
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| + | When put under a lead responsive promoter, the part is expected to show dosage dependent sequestration properties. | ||
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| + | ===Construction=== | ||
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| + | {|align="left" | ||
| + | ! [[file:pbrD_Gel.jpeg|200px|]] | ||
| + | |} | ||
| + | The part was artificially synthesised with corresponding primers that have overlap with GFP sequence. This allows it to be put downstream of the lead dependent biosensor (<a href="https://parts.igem.org/Part:BBa_K1758333">BBa_K1758333</a>). This part has been synthesised with a strong Ribosome Binding Site (<a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>) upstream of the coding sequence. | ||
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Revision as of 17:08, 21 October 2019
Lead Sequestration Protein with RBS
pbrD is a protein that sequesters lead. It is present downstream of a strong RBS (BBa_B0034) and binds to lead ions in the cytoplasm and decrease its concentration.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 532
- 1000COMPATIBLE WITH RFC[1000]