Difference between revisions of "Part:BBa K2974700:Design"
Monicacho123 (Talk | contribs) |
Monicacho123 (Talk | contribs) |
||
| Line 10: | Line 10: | ||
<center> | <center> | ||
| − | [[File:T-- | + | [[File:T--Lambert GA--Snapgene1.png|400px]] |
</center> | </center> | ||
<center><i>Figure 1: SnapGene design of BBa_J23100 Toehold eGFP construct.</i></center> | <center><i>Figure 1: SnapGene design of BBa_J23100 Toehold eGFP construct.</i></center> | ||
<center> | <center> | ||
| − | [[File:T-- | + | [[File:T--Lambert GA--NuPACK1.png|400px]] |
</center> | </center> | ||
<center><i>Figure 2: DeNovo Toehold Switch designed by the Collins Lab and visualized by the NuPack software. Lambert iGEM obtained this sequence via the Styczynski Lab at the Georgia Institute of Technology.</i></center> | <center><i>Figure 2: DeNovo Toehold Switch designed by the Collins Lab and visualized by the NuPack software. Lambert iGEM obtained this sequence via the Styczynski Lab at the Georgia Institute of Technology.</i></center> | ||
Revision as of 16:34, 21 October 2019
Strong Promoter (BBa_J23100) Toehold GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 728
Design Notes
Using SnapGene software, the LacZ Reporter Gene (BBa_K2550201) was identified and deleted from the T7 Promoter Toehold Ribosome Switch with LacZ expression (BBa_K2550000), then replaced with and replaced with enhanced Green Fluorescent Protein (BBa_E0040). The De-Novo Toehold Switch sequence (BBa_K2550100) was obtained from the Collins Lab at the Massachusetts Institute of Technology and was visualized through the NuPACK software.
Source
Green, A., Silver, P., Collins, J., & Yin, P. (2014). Toehold Switches: De-Novo-Designed