Difference between revisions of "Part:BBa K3051001"

Revision as of 19:47, 21 October 2019

Candida Antarctica Lipase A

Candida Antartica Lipase A (CALA) is a lipase extracted from the yeast Candida Antartica. It has been shown to work at very low temperatures. CALA has been shown to be very receptive to C8 p nitrophenol esters, compared to C4 and >C12 esters.

It has also been shown to be very sensitive to phenylmethylsulfonyl fluoride and high alcohol contents, causing a decrease in activity of as low as 50% compared to its control.[1]

It has a kDa of 49.3 and has been shown to react with p nitrophenol octanoate, to give p nitrophenol. Its molar extinction coefficient in water has been calculated to be 54570 M-1cm-1 (using ProtoPram ExPaSy tool). Its 3D structure has been calculated to be as follows:

20191020221459%21T--Warwick--CALA_3D_structure.png

Sources 1. Lan, Dong-Ming et al. “A novel cold-active lipase from Candida albicans: cloning, expression and characterization of the recombinant enzyme.” International journal of molecular sciences vol. 12,6 (2011): 3950-65. doi:10.3390/ijms12063950

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3051001 short</partinfo>
-Candida Antartica Lipase A (CALA) is a lipase extracted from the yeast Candida Antartica. It has been shown to work at very low temperatures. CALA has been shown to be very receptive to C8 p nitrophenol esters, compared to C4 and >C12 esters.
+<div style="width:50%">Candida Antartica Lipase A (CALA) is a lipase extracted from the yeast Candida Antartica. It has been shown to work at very low temperatures. CALA has been shown to be very receptive to C8 p nitrophenol esters, compared to C4 and >C12 esters.
 It has also been shown to be very sensitive to phenylmethylsulfonyl fluoride and high alcohol contents, causing a decrease in activity of as low as 50% compared to its control.[1]
-It has a kDa of 49.3 and has been shown to react with p nitrophenol octanoate, to give p nitrophenol. Its molar extinction coefficient in water has been calculated to be 54570 M-1cm-1 (using ProtoPram ExPaSy tool). Its 3D structure has been calculated to be as follows:
+It has a kDa of 49.3 and has been shown to react with p nitrophenol octanoate, to give p nitrophenol. Its molar extinction coefficient in water has been calculated to be 54570 M-1cm-1 (using ProtoPram ExPaSy tool). Its 3D structure has been calculated to be as follows:  </div>
 https://2019.igem.org/wiki/images/archive/1/14/20191020221459%21T--Warwick--CALA_3D_structure.png