Difference between revisions of "Part:BBa K274120:Experience"

Revision as of 16:29, 21 October 2019

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K274120

User Reviews

Characterization by UiOslo_Norway 2019

This part was of special interest to our team. The part consists of homologs of the genes we used; crtE, crtB and crtI from Deinococcus radiodurans. BBa_K274120 has individual ribosomal binding sites for each individual enzyme. The part that we made had a single ribosomal binding site followed by all three genes in a single open reading frame. The first two genes had their stopcodons removed (see BBa_K2971004 and BBa_K2971010). Figure 1 shows the result of expressing BBa_K274120 in E.coli (BL21) for 24 hours. The red coloration in the induced pellet is caused by the presence of lycopene. The induced culture with BBa_K274120 produced significant amount of lycopene when compared to expression of BBa_K2971004 (figure 2).

Figure 1: Induction of BBa_K274120 in Escherichia coli (BL21)
Expression was induced in 2% arabinose
From left to right :Uninduced E.coli with empty expression vector, Induced E.coli with empty expression vector, uinduced E.coli with BBa_K274120, induced E.coli with BBa_K274120.

Figure 2: Expression of crtEBI in Escherichia coli (BL21)
Expression was induced in 2% arabinose
From left to right :Uninduced E.coli with empty expression vector, Induced E.coli with empty expression vector, induced E.coli with crtEBI expression vector, uninduced E.coli with crtEBI expression vector.

UNIQcb0dd23118065226-partinfo-00000001-QINU UNIQcb0dd23118065226-partinfo-00000002-QINU