Difference between revisions of "Part:BBa K3183001"
Line 33: Line 33:
  
 
<br>
 
<br>
−
<br>
+
===References===
−
<div class="panel-group">
+
1. Kim, S F et al. “Cloning and nucleotide sequence of the Lactobacillus casei lactate dehydrogenase gene.” Applied and environmental microbiology vol. 57,8 (1991): 2413-7.<br>
−
  <div class="panel panel-default">
+
2. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.
−
    <div class="panel-heading">
+
−
      <h4 class="panel-title">
+
−
      </h4>
+
−
    </div>
+
−
    <div id="map" class="panel-collapse collapse">
+
−
      <div class="panel-body">
+

Revision as of 19:58, 21 October 2019


Surface (S)-Layer Protein Constitutive Promoter for Lactobacillus sp.

P-slp is a constitutive promoter which can be used in Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species.

This part encodes the promoter of the surface level protein gene in lactic acid bacteria. The (S)-layer protein (slp) promoter is derived from Lactobacillus acidophilus, and is responsible for the high level of transcription of stable mRNAs coding the S-protein monomers (Boot & Pouwels, 1996; Boot et al., 1996). It was characterised in six strains of Lactobacillus reuteri and Lactococcus lactis spp. cremoris MG1363 by Lizier et al, 2010.
The promoter shows good activity in L. lactis spp. cremoris MG1363, but has very little activity in L. reuteri.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Use by Team Oxford 2019

This part was used in our detection system to express AgrA2 and AgrC2 in L. reuteri. The composite part is: BBa_K3183102.


References

1. Kim, S F et al. “Cloning and nucleotide sequence of the Lactobacillus casei lactate dehydrogenase gene.” Applied and environmental microbiology vol. 57,8 (1991): 2413-7.
2. Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri Strains.” FEMS Microbiology Letters, vol. 308, no. 1, 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.