Difference between revisions of "Part:BBa K3183010"

Revision as of 16:16, 21 October 2019

mClover3 Fluorescent Protein, Codon Optimized for L. reuteri

mClover3 is a green fluorescent protein derivative which has been codon optimized for Lactobacillus reuteri 10023C, and may have uses in other Lactobacillus species.

Figure 1: mClover3 spectrum

Figure 2: - ldh promoter FI and OD600 time dependence - Blank corrected Fluorescence intensity and OD600 was plotted against time for ldh promoter. A large peak in OD600 can be observed, which could be an outliar due to random error in the instrument. Error bars represent Standard error of the mean. n=3

Fluorescence wavelengths

Bajar et al report the following excitation and emission data for mClover3 -

• Excitation max - 506nm
• Emission max - 518nm

Sequence and Features

Characterisation

This part was characterised in the composite part BBa_K3183101. Data for this part is shown below.